Molecular Biology

Molecular Biology

Molecular Biology is the study of how molecules interact with one another in living organisms to perform the functions of life. Mostly focusing on genes and proteins, molecular biologists use techniques like the polymerase chain reaction (PCR) to amplify DNA for various applications such as cloning, gene expression analysis (RT-PCR, RT-qPCR), Next Generation Sequencing (NGS) and more. Here you can find information on how Gilson instruments can help in this important research.

Normalization of DNA and RNA Samples with PIPETMAX® Normalization Assistant


 

keyword: PIPETMAX, Nucleic Acid Normalization, Normalization Workflow, Genomic DNA, cDNA, RNA, Isolation and Purification of Nucleic Acids, Normalization, Bovine DNA,


Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC

 

keyword: Oligonucleotides, qPCR, Next Generation Sequencing, Microarray Analysis, GX-271, Purification, TRILUTION LC


Preparation of Urine Samples for HPLC-MS/MS Detection of HNPAA Using the Gilson PIPETMAX® 268


 

keyword: PIPETMAX 268, HPLC-MS/MS, HNPAA, Metabolites. TNT, Sample Preparation


Automating the Promega GoTaq® PCR Core System II Reaction Using the Gilson PIPETMAX® 268


 

keyword: Promega GoTaq PCR Core System II, PIPETMAX 268, Plasmid DNA


High-Resolution Purification of Sheared Lambda Phage DNA Using a Gilson HPLC System


 

keyword: High-Performance Liquid  Chromatography (HPLC), DNA Shearing, DNA Separation, Methylation, Lambda  Phage, Gradient Pattern, Electrophoresis, Next-Generataion Sequencing  (NGS)


Automated Patient Barcode Tracking and Sample Preparation Using the Gilson GX-281 Barcode Matching System


 

keyword: GX-281 Liquid Handler, Sample  Tracking, Barcode Matching, Laboratory Information Management System  (LIMS), Laboratory Informatics, Sample Preparation, Automation, Clinical  Laboratory, Patient Samples


Selecting the Proper Pipetting Tools for Optimal PCR and qPCR in the Laboratory


             

keyword: PCR, qPCR, Pipetting, Biomolecule, Disposable Tips, Accuracy, PIPETMAN M, MICROMAN, PIPETMAN L, GmCLab


Efficient LC-MS/MS Method for Determination of delta9-Tetrahydrocannabinol in Human Plasma Using Solid Phase Extraction Clean-up


 

keyword: SPE, Tetrahydrocannabinol, Metabolites, Human Plasma, LC-MS/MS, LC-MS, GC-MS, MS


Methamphetamine and Amphetamine Drug Screening in Urine via Solid Phase Extraction


 

keyword: SPE, Urine Analysis, Drug Screening, mAMP, AMP


Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using The Gilson PIPETMAX®

 

keyword: Next Generation Sequencing, NGS, Sample Prep, DNA Library Prep, Automation, NxSeq, PIPETMAX


Determination of t, t-Muconic Acid in Urine via Automated Sample Preparation and HPLC

keyword: Gilson GX-271 ASPEC with Dual Syringe Pump, TRILUTION LH v3.0 Liquid Handling Software, Solid Phase Extraction, SPE, Gilson GX-271 Liquid Handler, TRILUTION LC v2.1 Liquid Chromatography Software, human biomonitoring kit


Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually using Gilson PIPETMAN® M Multichannel, PIPETMAN L, and Automated using GX-271 ASPEC® and GX-241 Liquid Handler

keyword: PIPETMAN® L, PIPETMAN M Multichannel, GX-241 Liquid Handler, GX-274 ASPEC™, Coomassie (Bradford) Protein Assay, Molecular Biology, Automation, Laboratory Equipment


An Undergraduate's Approach to Method Development: Chromatography Lessons Learned through Experimentation

keyword: PLC 2020, Purification, Preparative Chromatography, Reversed Phase Chromatography, Method Development, Sparation Science, Pharmaceuticals


Manual Automation of Rapid N-Glycan Sample Preparation - Reliable Results from Error Free Sample Tracking and Accurate Pipetting

keyword: N-Glycan, Glycobiology, hlgG, Sample Cleanup, Purification, Glycoanalysis, Microchromatography


Automation of Solid Phase Extraction (SPE) Multi-method for GC/MS Analysis of Drugs of Abuse in Human Serum

keyword: GX-271 ASPEC™, TRILUTION LH Liquid Handling Software, Solid Phase Extraction (SPE), Psychotropic drugs, Gas Chromatography Mass Spectrometry (GC/MS)


Improving Laboratory Efficiency & Flexibility with Automated Sample Preparation Systems - The Impact of the GX-281 Dual Z-Arm Weighing Station

keyword: Weighing Station, GX-281 Liquid Handler, Sample Preparation, Automated Systems, Bar Coding, Tube Gripper, TRILUTION LH v3.0 Liquid Handling Software


Automated Solid Phase Extraction (SPE) of Benzodiazepines from Urine using the Gilson GX-274 ASPEC®

keyword: Gilson GX-274 ASPEC™, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis,Diazepam, Benzodiazepines, Urine, Pharmaceuticals


Automated Solid Phase Extraction of THC and Metabolites from Whole Blood using the Gilson GX-274 ASPEC®

keyword: Gilson GX-274 ASPEC™, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis, Tetrahydrocannabinol (THC), Metabolites, Whole Blood


使用Gilson  Gilson GX-274和Gilson ASPEC™ XL自动检测尿样中的 VMA, HVA和5-HIAA

keyword: Gilson GX-274, TRILUTION® LH, Catecholamines, Urine, Vanillylmandelic acid (VMA), Homovanilic Acid (HVA), 5-hydroxyindoleacetic Acid (5-HIAA), Tumors, Diagnosis, Biosyntheses, Recipe


Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling

keyword: Automation, Biomarkers, Glycans, Glycomics, GX-274 ASPEC, Human Serum, Mass Profiling, Mass Spectrometry, MALDI-FTIR, MS, N-Glycan, N-linked Glycans, Serum, Solid Phase Extraction, SPE


An Automated Liquid Handling Strategy for Complex Matrices Based on Sample Viscosity

keyword: Accuracy, Automated Liquid Handling, Beverages, Blood, GX-271, Liquid Handling, Liquid Transfer, Low Volume Dispensing, Optimization, Performance, Pipetting


Identification and Qualification of Illegal Drugs and Benzodiazepines in Human Plasma by LC/MS-MS Analysis after Solid Phase Extraction (SPE)

keyword: Amphetamines, Benzodizepines, Blood, Cocaine, Forensics, GX-271 ASPEC, Human Plasma LC/MS-MS, Opiates, Plasma, Solid Phase Extraction (SPE)


Automated Method for Dispensing Fruit Fly Food into Culture Vials and Bottles

keyword: 281 Liquid Handler, Drosophila, Melanogaster, Fly Food, Fruit Fly, Genetics, Developmental Biology, Animal Models, Medical Research, Media, Culturing


Automated Fractionation System that Increases Recovery and Optimizes Precision of Solid Phase Extraction Methodology

keyword: Automated Fractionation, SPE, Serum, Plasma, Urine


Automated SPE Method for the Determination of Cotinine in Biological Fluid

keyword: Cotinine, Nicotine, GX-271 ASPEC/406 Dilutor, SPE


An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thiorizidine from Plasma

keyword: Neuroleptic drugs, SPE, SPE 215


An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen from Plasma

keyword: SPE, Ketoprofen, Ibuprofen, Prostaglandin


Accessing the E-Gel® 96 System via the Quad-Z 215 with Disposable Tips

keyword: QUAD-Z 215, Disposable Tips, E-Gel 96 gels, Agarose, DNA Electrophoresis, DNA Fragment


Quad-Z 215 with Disposable Tips: Implementing Use of ZipTips® for Purification of Bio Samples

keyword: QUAD-Z 215, Disposable Tips, ZipTips, Plasma, Biological Samples, Solid Phase Extraction (SPE), Purification, Concentration, Peptides, Proteins, Oligonucleotides


Reformatting Procedures Employing a Quad-Z 215 with Disposable Tips

keyword: QUAD Z-215, Disposable Tips, Microplate Reformatting, PCR Preparation, Protein Crystallography, Microplate Replication

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