331/332 HPLC Pumps
The dual-piston, multi-solvent 331/332 Pumps are a reliable and affordable option for Gilson's semi-preparative HPLC systems. The 333/334 Pumps are the ideal choice for preparative HPLC applications in normal and reverse-phase modes. All pumps offer multi-solvent selection, with an optional four-solvent valve that allows up to eight solvents. The pumps feature a variable-volume, dynamic mixer for optimized gradient performance.
333/334 Pumps - The Pumping Choice for Preparative HPLC
The 333/334 Pumps provide a preparative pumping solution to accommodate columns ranging from 10 mm to 50 mm ID in high pressure normal and reverse phases at flow rate from 1 mL/min to 200 mL/min and pressures up to 3040 psi (210 bar). Use milligram to gram injection levels - up to 15 g depending on column size and loading capacity. With additional pumps in parallel, you can double the flow rate up to 400 mL/min. For isocratic methods, choose the 333 master pump; choose the combination of the 333 and 334 Pumps for binary methods. Control 333/334 Pumps and an entire preparative HPLC system via TRILUTION® LC software. With the 333 Pump as master pump, control:
• Flow-rate gradient
• Four-solvent selection and step gradient (additional valve)
• Composition gradient (one or two additional pumps)
• Sample-injection pump
• UV/VIS detector
331/332 Pumps - The Pumping Choice for Semi-Preparative HPLC
The 331/332 Pumps accommodate flow rates ranging from 0.5 mL/min to 50 mL/min at pressures up to 8700 psi (600 bar) using columns up to 30 mm ID. The pumps handle milligram-level of sample mass throughput per injection at >50 mg with a 20 mm ID column. Control begins with a 331 master pump. Keypad controls include safety files, anomaly processing procedures, and GLP functions. TRILUTION® software provides control within a complete prep workstation and includes data processing and results reporting.
Specifications
| 331&332 Pump | ||
| Manufacturing Standards | Meet applicable Safety and EMC certification standards; UL and CE certified | |
| Composition and Mixing | Increment: 0.1% | |
| Dwell Volume: 372–2,372 µL, pressure independent | ||
| Gradient Linearity Range: 1–98% B for 10 mL/min and higher | ||
| Environmental Conditions | Indoor use | |
| Altitude: up to 2000 m | ||
| Temperature range: 5–40°C | ||
| Air pressure: 75–105 kPa | ||
| Pollution degree: 1 or 2, in accordance with IEC 66 | ||
| Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C | ||
| Front Panel (331 Pump) | 40-character, eight-line display, adjustable contrast and screen saver; 25 functional keys, including six soft keys | |
| Flow Rate | 0.5–50 mL/min (8,700 psi) | |
| Selectable units: µL/min and mL/min (default: mL/min) | ||
| Adjustment increment: 1 µL/min | ||
| Accuracy: bias <±1% or 1.3 µL/min (331), bias <±2% or 13 µL/min (331/332) | ||
| Repeatability: RSD <0.5% or 1 µL/min (331), RSD <0.7% or 7 µL/min (331/332) | ||
| Liquid-Contact Materials | 316L stainless steel, titanium, FEP, PTFE, PCTFE, ETFE, PEEK, UHMWPE, ruby, sapphire, and ceramics | |
| Power Requirements | Frequency: 50–60 Hz | |
| Voltage: 90–120 or 220–260V; mains voltage fluctuations not to exceed ±10% of the nominal voltage | ||
| Pressure | Nominal range: from 0.5 MPa (5 bar, 70 psi) up to 60 MPa (600 bar, 8,700 psi) | |
| Pump Options | 331: single-solvent master pump (w/control panel), 0.05–50 mL/min, 60 MPa (8,700 psi) | |
| 331/332: two-solvent pumping system, 0.5–50 mL/min, 60 MPa (8,700 psi) | ||
| 332: third-solvent, remote controlled pump (w/o control panel), 0.5–50 mL/min, 60 MPa (8,700 psi) | ||
| Software | Controlled by TRILUTION® LC Software or by the front panel display on the 331 | |
| Dimensions (w x d x h) | 331: 26 x 41 x 50.7 cm (10.2 x 16.1 x 20 in.) | |
| 332: 26 x 41 x 38.7 cm (10.2 x 16.1 x 15.2 in.) | ||
| Weight | 35 kg (77 lbs.) | |
Supporting Literature
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