Pharmaceutical

Pharmaceutical

Gilson, Inc., a global leader in preparative HPLC, offers a full line of chromatographic instruments to suit the needs of today’s scientist in the purification market. From robust instruments for reformatting techniques to complicated assay procedures with disposable tip or fixed probe options, Gilson has an automated solution to meet your liquid handling application requirements.

Purification of Vanillin by a Combination of Flash and Preparative HPLC with the PLC 2250

keyword: Vanillin, Vanilla Extract, PLC Purification Systems, FLASH Chromatography, Preparative HPLC.

 


Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC

keyword: Oligonucleotides, qPCR, Next Generation Sequencing, Microarray Analysis, GX-271, Purification, TRILUTION LC

 


Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System

keyword: High-Performance Liquid  Chromatography (HPLC), Mass Spectrometry (MS), Preparative HPLC/MS, Fraction Collection, Fraction Reinjection, Probenecid, TRILUTION LC, Conditional Logic Fraction Collection

 


Evaluation of Ketone-Containing Terpene Compounds Using Evaporative Light Scattering Detection

keyword: PrepELS™II Detector, Evaporative Light Scattering (ELS), High-Performance Liquid Chromatography (HPLC), Terpenes, Ketones, Thujone, Camphor, Essential Oil Constituents, Volatile Compounds

 


Automating the Promega GoTaq® PCR Core System II Reaction Using the Gilson PIPETMAX® 268

keyword: Promega GoTaq PCR Core System II, PIPETMAX 268, Plasmid DNA

 


High-Resolution Purification of Sheared Lambda Phage DNA Using a Gilson HPLC System

keyword: High-Performance Liquid Chromatography (HPLC), DNA Shearing, DNA Separation, Methylation, Lambda Phage, Gradient Pattern, Electrophoresis, Next-Generataion Sequencing (NGS)

 


Automated Patient Barcode Tracking and Sample Preparation Using the Gilson GX-281 Barcode Matching System

keyword: GX-281 Liquid Handler, Sample Tracking, Barcode Matching, Laboratory Information Management System (LIMS), Laboratory Informatics, Sample Preparation, Automation, Clinical Laboratory, Patient Samples

 


Recovery and Purification Comparison of Glycinin Using Various pH Extraction Procedures on a Continuous Injection Gradient Gilson PLC 2020 System

keyword: Glycinin, Soy Protein, Beta Conglycinin, PLC 2020 Personal Purification System, Semi-Preparative Purification, Extraction, HPLC, Continuous Injection, Large Volume Loading, Automated Loading Pump, Gradient

 


Purification of Aglain Natural Products with the Gilson PLC 2020 Personal Purification System

keyword: Aglain, Natural Product HPLC Purification, Synthesis, PLC 2020

 


Isolation of Synthetic Pleiomaltinine Using the Gilson PLC 2020 Personal Purification System

keyword: Pleomaltinine, Synthetic HPLC Purification, PLC 2020, Biologically Active, Pyrone

 


Selecting the Proper Pipetting Tools for Optimal PCR and qPCR in the Laboratory

keyword: PCR, qPCR, Pipetting, Biomolecule, Disposable Tips, Accuracy, PIPETMAN M, MICROMAN, PIPETMAN L, GmCLab

 


Determination of Pesticides in Drinking Water via LC-MS/MS Using Polymeric Solid Phase Extraction Cartridges

keyword: SPE, Pesticide, Herbicide, Fungicide, Pharmaceuticals, Drinking Water, WHO, National Standards

 


Efficient LC-MS/MS Method for Determination of delta9-Tetrahydrocannabinol in Human Plasma Using Solid Phase Extraction Clean-up

keyword: SPE, Tetrahydrocannabinol, Metabolites, Human Plasma, LC-MS/MS, LC-MS, GC-MS, MS

 


Methamphetamine and Amphetamine Drug Screening in Urine via Solid Phase Extraction

keyword: SPE, Urine Analysis, Drug Screening, mAMP, AMP

 


Optimizing Chiral Separations and Purification of Enantiomers through Selective Sample Slicing and Column Switching

keyword: Enantiomers, Purification, Chiral Pharmaceuticals, GX-271 Semi-Preparative HPLC System, Column Switching, Sample Slicing, Sample Cleanup

 


Natural Product Purification: Bioactive trans-Cinnamaldehyde from Zingiberaceae Extract for Use as a Skin Lightening Agent

keyword: Cosmetics, Natural Products, Zingiberaceae, trans-Cinnamaldehyde, GX-281 Preparative Purification System, Biologically Active Compounds, High Pressure Liquid Chromatography (HPLC)

 


High Loading Purification of a Ribonuclease A Protein Using a Manual Purification System

keyword: Ribonuclease A (RNAse), Protein, Manual HPLC Purification, Bovine Pancreas, Ion Exchange, High Loading Purification

 


Purification of a Protein Using the PLC 2020 Personal Purification System

keyword: Protein, Anion Exchange, Purification, Gilson PLC 2020

 


Purification of the B-Lactoglobulin A Protein with the Gilson GX-271 LC/MS Purification System

keyword: B-Lactoglobulin, Protein, Mass-Based Purification, Target Ions, Gilson LC/MS System

 


Scale-Up of a 6-mer Synthesized Peptide Using a Manual HPLC System

keyword: Peptide, Scale-Up Purification, Gilson Manual System

 


Preparative Purification of a Synthesized Peptide Using an Automated LC/MS Purification System

keyword: Peptide, Mass-Based Purification, Target Ions, Gilson LC/MS System

 


Purification of a 25-mer ssDNA Oligonucleotide Using the PLC 2020 Personal Purification System

keyword: Single Stranded DNA, Oligonucleotides, Purification, Gilson PLC 2020

 


Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually using Gilson PIPETMAN® M Multichannel, PIPETMAN L, and Automated using GX-271 ASPEC® and GX-241 Liquid Handler

keyword: PIPETMAN® L, PIPETMAN M Multichannel, GX-241 Liquid Handler, GX-274 ASPEC®, Coomassie (Bradford) Protein Assay, Molecular Biology, Automation, Laboratory Equipment

 

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An Undergraduate's Approach to Method Development: Chromatography Lessons Learned through Experimentation

keyword: PLC 2020, Purification, Preparative Chromatography, Reversed Phase Chromatography, Method Development, Sparation Science, Pharmaceuticals

 

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Manual Automation of Rapid N-Glycan Sample Preparation - Reliable Results from Error Free Sample Tracking and Accurate Pipetting

keyword: N-Glycan, Glycobiology, hlgG, Sample Cleanup, Purification, Glycoanalysis, Microchromatography

 

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Urification and Analysis of Bioactive Extracts from Marine Yeast with the Gilson Semi-Micro 2D HPLC System

keyword: 2D HPLC (2 Dimensional High Pressure Liquid Chromatography), TRILUTION LC Liquid Chromatography Software, Gilson 233XL, Bioactive, Marine Yeast

 

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Automated Separation of Crude Extracts from Natural Products into Fractions for Primary High Throughput Screening

keyword: Gilson 215 Liquid Chromatography System, TRILUTION LC Liquid Chromatography Software, High Throughput Screening, Natural Products

 

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Natural Product Purification of Lycopene and B-Carotene Using Flash Column Sample Clean-up Prior to High Pressure Normal Phase Chromatography on the Gilson PLC 2020

keyword: PLC 2020, Natural Product Purification, Flash Chromatography, Lycopene, Tea, B-Carotene, Tomatoes

 

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A Quick Study - Comparison of Chromatography Normal Phase vs. Flash on the Gilson PLC 2020

keyword: Purification, Normal Phase Chromatography, Flash Chromatography, PLC 2020, Preparative Chromatography

 

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Improving Laboratory Efficiency & Flexibility with Automated Sample Preparation Systems - The Impact of the GX-281 Dual Z-Arm Weighing Station

keyword: Weighing Station, GX-281 Liquid Handler, Sample Preparation, Automated Systems, Bar Coding, Tube Gripper, TRILUTION LH v3.0 Liquid Handling Software

 

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Automated Solid Phase Extraction (SPE) of Benzodiazepines from Urine using the Gilson GX-274 ASPEC®

keyword: Gilson GX-274 ASPEC®, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis,Diazepam, Benzodiazepines, Urine, Pharmaceuticals

 

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A Practical User Guide for the Determination of Optimal Purification Gradients for the Gilson PLC 2020

keyword: Purification, Pharmaceuticals, Optimal Solvent Gradients, Isolation Gradients, Organic Compounds, PLC 2020, Preparative Chromatography

 

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Automated Determination of VMA, HVA, 5-HIAA, in Urine Using the Gilson GX-274 and Gilson ASPEC® XL

keyword: Gilson GX-274, TRILUTION® LH, Catecholamines, Urine, Vanillylmandelic acid (VMA), Homovanilic Acid (HVA), 5-hydroxyindoleacetic Acid (5-HIAA), Tumors, Diagnosis, Biosyntheses, Recipe

 

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Rapid Isolation of Geraniin from Nephelium Lappaceum Rind Waste Using the Gilson GX-281 Preparative HPLC Purification System

keyword: Gilson GX-281 Preparative HPLC System, TRILUTION® LC, Geraniin, Alpha Glucosidase, Alpha Amylase, Aldol Reductase, AGE, Nephelium Lappaceum L., University of Malaysia, Anti-Hyperglycemic Agent, Hyperglycemia, Purification, Natural Products, HPLC, Diabetes

 

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Identifying and Collecting Weak UV Absorbance and Low Level Peaks of Interest From a Complex Mixture Using Conditional Logic Purification on Mass and UV Signals

keyword: Gilson GX-271 LC/MS Purification System, Fraction Collection, Active Pharmaceutical Ingredient (API), Metoprolol, Verapamil, Chloramphenicol, Doxepin, Sulfamethazine

 

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Optimizing the Purification of a Standard Chrial Compound Utilizing a Benchtop, Multi-Purpose, Semi-Preparative to Preparative HPLC System

keyword: Chiral, Enantiomer, HPLC, Normal Phase Chromatography, PLC 2020 Personal Chromatography System, Preparative Chromatography, Purification, Resolution, Trans-Stilbene Oxide

 

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Automated Solid Phase Extraction of Allantoin from Cosmetics and Topical Pharmaceuticals Prior to Analysis by HPLC

keyword: Allantoin, Automation, CHROMABOND HR-XA, Cosmetics Glyoxylic Acid Diureide, GX-271 ASPEC, HILIC, HPLC, Ointments, Pharmaceuticals, Sample Preparation, Sold Phase Extraction, SPE, Topical Pharmaceuticals, 5-Ureidohydantoin

 

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Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling

keyword: Automation, Biomarkers, Glycans, Glycomics, GX-274 ASPEC, Human Serum, Mass Profiling, Mass Spectrometry, MALDI-FTICR, MS, N-Glycan, N-linked Glycans, Serum, Solid Phase Extraction, SPE

 

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FTLC Fraction Trapping Liquid Chromatography

keyword: HPLC, Preparative Chromotography, Gilson FT-LC System

 

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Automated Fractionation System that Increases Recovery and Optimizes Precision of Solid Phase Extraction Methodology

keyword: Solid Phase Extraction, SPE, Serum, Plasma, Urine

 

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A Novel Approach to GX-281 Semi-Preparative System Purification of Natural Compounds Using Isolation Gradients

keyword: GX-281 Purification System, TRILUTION LC, natural compounds, Oregano, Rosmarinic Acid, Carvacol, isolation gradient, purification, fraction collection

 

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Systematic Look at the Affects of Various Solvents, Injection Techniques and Sample Amounts on Preparative HPLC Columns

keyword: Various Solvents, Injection Techniques, Solvents

 

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A Totally Automated Solution for Normal and RP Preparative HPLC with Analytical Purification Determination: cLC

keyword: Analytical Purification Determination, cLC  System, Reverse-Phase (RP) Chromatography, Normal Phase (NP) Chromatography

 

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A Compact Automated Liquid Handler for Microbatch Protein Crystallography

keyword: Protein Crystallization, Microbatch

 

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A Multipurpose Preparative System for the Detection and Collection of Active and Non-active UV/VIS Compounds

keyword: Active and Non-Active UV/VIS Compounds, ELSD

 

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An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thiorizidine from Plasma

keyword: Neuroleptic Drugs, SPE, SPE 215

 

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Fast PCLC: A Widely Application Technique for Laboratory Scale Preparative Separations

keyword: Fast PCLC

 

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An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen from Plasma

keyword: SPE, Ketoprofen, Ibuprofen, Prostaglandin

 

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The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Foods and Dietary Supplements

keyword: Ephedra, Norephedrine, Pseudoephedrine, Methylephedrine, Dietary Supplements, Nutraceuticals, Gilson SPE 215

 

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Automated Preparation of MALDI-TOF Plates via a Spring-Loaded Probe

keyword: 223 Sample Changer, Matrix Assisted Laser Desorption/Ionization (MALDI), peptide, MALDI-TOF MS, bradykinin, angiotensin II, ACTH, a-cyano-4-hydroxycinnamic acid, Bruker AnchorChip

 

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